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LSS4 Cryo-EM of macromolecular assemblies

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Introduction to 2D and 3D Image Processing and Analysis
Organizers: Sidnei Paciornik (PUC-Rio, Brazil) and Karen Soares Augusto (ZEISS)
Introduction to Scanning Electron Microscopy
Organizer: Fabiano Emmanuel Montoro (LNNano – Laboratório Nacional de Nanotecnologia)
Introduction to Transmission Electron Microscopy
Organizers: Jefferson Bettini (LNNano, Brazil) and Daniel Lorscheitter Baptista (UFRGS, Brazil)
CellProfiler course – Learn to turn bioimages into data
Organizer: Mario Costa Cruz (USP, Brazil)
The molecular revolution - Introduction to cryoEM Single Particle Analysis
Organizers: Leonardo Talachia Rosa (USP, Brazil) and Germán Sgro (USP, Brazil)
(On-line): Tratamento de imagens e colorização pelo Photoshop - curso básico: demonstrativo e prático
Organizer: Dra. Marlene Benchimol (UFRJ, Brazil)
Lunch
The molecular revolution - Introduction to cryoEM Single Particle Analysis
Organizers: Leonardo Talachia Rosa (USP, Brazil) and Germán Sgro (USP, Brazil)
Introduction to 2D and 3D Image Processing and Analysis
Organizers: Sidnei Paciornik (PUC-Rio, Brazil) and Karen Soares Augusto (ZEISS)
Introduction to Scanning Electron Microscopy
Organizers: Fabiano Emmanuel Montoro (LNNano, Brazil)
Introduction to Transmission Electron Microscopy
Organizers: Jefferson Bettini (LNNano, Brazil) and Daniel Lorscheitter Baptista (UFRGS, Brazil)
Lunch
Bridging Borders in BioImaging: A Global Workshop on Advancing Imaging - Technologies through your Network and Imaging Communities
Andrés Olivera (LABI), Johanna Bischof (EURO-BioImaging), Karina Alleva (CEBEM), Kildare Miranda (LABI) and Yara Reis (Global Bioimaging)
Registration
OPEN PLENARYChairs: Rodrigo Prioli Menezes (PUC) - Brazil
  • PT-1.1
    18:30 - 19:30
    4E2S - New paradigms for the study of nanomaterials by advanced TEM methods
    Daniel Ugarte
    Room: GERTRUDE REMPFER
Welcome Reception
PLENARY TALK 2Chairs: Guillermo Solórzano-Naranjo
  • PT-2.1
    09:00 - 10:00
    4E2T - The Use of Correlative Microstructural Characterization to Advance Our Understanding of Irradiation Damage in Steels – A Metallurgist’s Perspective
    Mary Grace Burke
    Room: GERTRUDE REMPFER
Coffee Break and Exhibition
Lunch
Elevator Pitch 1
PLENARY TALK 3Chairs: Paul Kotula (SANDIA NATIONAL LABS) - United States
  • PT-3.1
    14:20 - 15:20
    4E2V - The TOMO Project – Integrating a Fully Functional Atom Probe in an Aberration-Corrected TEM
    Joachim Mayer
    Room: GERTRUDE REMPFER
POSTER SESSION I
  • PS-I.LSS4.49
    17:30 - 18:30
    4DNY - Solving Protein Complexes: Producing Good Septin Complexes for Electronic Microscopy Analyses
    Heloisa Ciol; Deborah Cesar Mendonça; Giovanna Christe dos Reis Saladino; Luis Alberto Valverde Fernández; Richard Charles Garratt; Ana Paula Ulian de Araujo
    Room: Exhibition Area
  • PS-I.LSS4.50
    17:30 - 18:30
    4DQZ - Towards the structural characterization of the secretory tube of the Type VI Secretion System of Klebsiella pneumoniae by Single Particle Cryoelectron Microscopy
    Maria Eduarda Ferreira do Rosário; Débora Caroline Amorim de Souza; Antonio Carlos Borges; Mariana Araújo Ajalla Aleixo; Rodrigo Portugal; Marcel Menezes Lyra da Cunha; Gisele Cardoso de Amorim
    Room: Exhibition Area
CIASEM ASSEMBLY
Chair: Paul Kotula, USA
Happy Hour
Confraternization
PLENARY TALK 4Chairs: Kildare Miranda (UFRJ) - Brazil
  • PT-4.1
    09:00 - 10:00
    4E2W - Global Bioimaging Networks are Changing the Science Ecosystem
    Claire Brown
    Room: GERTRUDE REMPFER
Coffee Break and Exhibition
LIFE SCIENCES SYMPOSIUM IVChairs: Marin van Heel (CNPEM) - Brazil; Rodrigo Portugal (CNPEM) - Brazil
  • LSS4.1
    10:20 - 10:50
    4E6E - Cryo-EM reveals structures and activities of membrane remodeling proteins (Invited Lecture)
    Carsten Sachse
    Room: HERTHA MEYER
  • LSS4.2
    10:50 - 11:20
    4E6G - A Tale of Two Volumes, Developments in volume Electron Microscopy (Invited Lecture)
    Roland Fleck
    Room: HERTHA MEYER
Lunch
Elevator Pitch 2
PLENARY TALK 5Chairs: Rodrigo Portugal (CNPEM) - Brazil
  • PT-5.1
    14:20 - 15:20
    4E2X - ELECTRON CRYOTOMOGRAPHY OF CELLS: PROGRESS AND POTENTIAL
    Grant Jensen
    Room: GERTRUDE REMPFER
POSTER SESSION II
  • PS-II.LSS4.17
    17:30 - 18:30
    4DXP - Development of a Microfluidic Device for Sample Preparation for Time-Resolved Electron Cryo-Microscopy
    Daniela Cristina Serdan; Mariana Araújo Ajalla Aleixo; Luis Carlos Silveira Vieira; Maria Helena de Oliveira Piazzetta; Angelo Luiz Gobbi; Rodrigo Portugal
    Room: Exhibition Area
  • PS-II.LSS4.18
    17:30 - 18:30
    4DY6 - Structural Comparison of Arboviruses
    Katie Cristina Takeuti Riciluca; Sayan Bhakta; Rodrigo Portugal; Marin van Heel
    Room: Exhibition Area
  • PS-II.LSS4.19
    17:30 - 18:30
    4DRJ - Investigating transport and signalling networks in the re-emerging pathogen Bordetella pertussis
    Leonardo Talachia Rosa
    Room: Exhibition Area
  • PS-II.LSS4.20
    17:30 - 18:30
    4E24 - SARS-Cov-2 Spike Protein as a Model for Ligand Screening Using Cryo-EM
    Mariana Araújo Ajalla Aleixo; Matheus Pintor Baptista; Maxuel de Oliveira Andrade; Daniela Barretto Barbosa Trivella; Rodrigo Portugal
    Room: Exhibition Area
  • PS-II.LSS4.21
    17:30 - 18:30
    4DYB - Comparing all 3D Structures of SARS-CoV2 Spike proteins
    Sayan Bhakta; Katie Cristina Takeuti Riciluca; Rodrigo Portugal; Michael Schatz; Marin van Heel
    Room: Exhibition Area
Pint of Microscopy
Marcos Farina
Happy Hour
SBMM ASSEMBLY
Chair: Marco Guimarães (UFES)
PLENARY TALK 6Chairs: Hernandes Faustino de Carvalho (UNICAMP) - Brazil
  • PT-6.1
    09:00 - 10:00
    4E2Y - INTRAVITAL MICROSCOPY AS A POWERFUL TOOL TO STUDY LEUKOCYTE TRAFFICKING IN INFLAMMATION
    Hellen Ishikawa-Ankerhold
    Room: GERTRUDE REMPFER
Coffee Break and Exhibition
Lunch
Elevator Pitch 3
PLENARY TALK 7Chairs: Andre Linhares Rossi (CBPF) - Brazil
  • PT-7.1
    14:20 - 15:20
    4E2Z - Temporal and spatial scale evaluations in transmission electron microscopy: contributions to Biomineralization
    Marcos Farina
    Room: GERTRUDE REMPFER
Happy Hour
Awards and Closing Ceremony